Association of Glu298Asp Polymorphism of eNOS Gene with Cardiovascular Diseases

Molecular-genetic diagnostics of polygenic diseases is a new and interesting area in laboratory diagnostics, especially the cardiovascular diseases, as one leading causes mortality world population.
 Aims: The aim paper was to analyse variants endothelial nitric oxide synthase gene (NOS3) (Glu298Asp/G894T) human population Tuzla Canton relation diseases.
 Study Design: study included 112 respondents both sexes over 18 years old. experimental group for analysis polymorphism (Glu298Asp) 56 with disease (hypertension), while control comprised healthy without prior history (sample/control).
 Place Duration Study: Blood sampling performed at Medical Center "Plava Poliklinika", “Plava Group”, Department Biochemistry, Microbiology Genetics, Tuzla. DNA isolation molecular-genetic samples were Laboratory scientific research Biology, Faculty Natural Sciences Mathematics Tuzla.
 Methodology: genotyping eNOS Glu298Asp all determined by an optimized method based on PCR-RFLP reaction.
 Results: In total sample respondents, highest genotype frequencies recorded GG (53.5%) GT (35.7%). lowest frequency TT genotype, which 10.8%.
 Conclusion: results obtained provide good guidelines further association between high number candidates will contribute incorporation these into existing regional European genetic database.